A system for automated genome annotation

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چکیده

Eucalyptus globulus is a tree used to produce paper pulp. With the aim to study the genes responsible for wood formation in E. globulus, which are not yet fully understood although known to have an impact on the quality of paper pulp, GenEglobwq project was defined. One of the project goals is the development of an annotation module, to automatically annotate the gene sequences obtained. Therefore, the aim of this thesis was to extend a web information system, GEDI, used to manage the data from GenEglobwq project, in order to provide a tool for automated genome annotation. In this sense the annotation module in the GEDI system was created, which makes use of an automatic genome annotation pipeline, named MAKER, which can be used to annotate any type of organism. In order to validate the annotation module operation, the chloroplast genomes of Eucalyptus globulus and Eucalyptus grandis was annotated. However, like any automatic genome annotation pipeline, not all the genes were annotated, being necessary a manual curation of the results.

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تاریخ انتشار 2010